Journal: Pathogens

Article Title: Peptidases Are Potential Targets of Copper(II)-1,10-Phenanthroline-5,6-dione Complex, a Promising and Potent New Drug against Trichomonas vaginalis

doi: 10.3390/pathogens12050745

Figure Lengend Snippet: Parameters used for qRT-PCR of Trichomonas vaginalis peptidases.

Article Snippet: The analysis was performed on a G2-XS Q-TOF Xevo mass spectrometer ® and the identification was based on the genome database of T. vaginalis ( T. vaginalis G3, G3; ATCC PRA-98, WGS project AAHC01000000) held on the ProteinLynx Global Server (PLGS) platform version 2.2.5 and Uniprot database ( https://www.uniprot.org/ , accessed on 9 November 2019).

Techniques: Concentration Assay

Journal: Pathogens

Article Title: Peptidases Are Potential Targets of Copper(II)-1,10-Phenanthroline-5,6-dione Complex, a Promising and Potent New Drug against Trichomonas vaginalis

doi: 10.3390/pathogens12050745

Figure Lengend Snippet: Three-dimensional model for TvMP50 of Trichomonas vaginalis . ( A ) PIDDT values for the amino acid sequence of the structure modeled using Alphafold. ( B ) Active site, represented by the green marking. ( C ) Identification of amino acids from the enzyme’s active site. ( D ) Electrostatic charge on the surface of the TvMP50 calculated for pH values of 4 to 7. The red-white-blue scale refers to the minimum (−5 kT/e, red) and maximum (5 kT/e, blue) potentials of the surface.

Article Snippet: The analysis was performed on a G2-XS Q-TOF Xevo mass spectrometer ® and the identification was based on the genome database of T. vaginalis ( T. vaginalis G3, G3; ATCC PRA-98, WGS project AAHC01000000) held on the ProteinLynx Global Server (PLGS) platform version 2.2.5 and Uniprot database ( https://www.uniprot.org/ , accessed on 9 November 2019).

Techniques: Sequencing

Journal: Pathogens

Article Title: Peptidases Are Potential Targets of Copper(II)-1,10-Phenanthroline-5,6-dione Complex, a Promising and Potent New Drug against Trichomonas vaginalis

doi: 10.3390/pathogens12050745

Figure Lengend Snippet: Three-dimensional model for TvGP63 of Trichomonas vaginalis . ( A ) PIDDT values for the amino acid sequence of the structure modeled using Alphafold. ( B ) Active site, represented by the green marking. ( C ) Identification of amino acids from the enzyme’s active site ( D ) Electrostatic charge on the surface of the TvMP50 calculated for pHs 4 to 7. The red-white-blue scale refers to the minimum (−5 kT/e, red) and maximum (5 kT/e, blue) potentials of the surface.

Article Snippet: The analysis was performed on a G2-XS Q-TOF Xevo mass spectrometer ® and the identification was based on the genome database of T. vaginalis ( T. vaginalis G3, G3; ATCC PRA-98, WGS project AAHC01000000) held on the ProteinLynx Global Server (PLGS) platform version 2.2.5 and Uniprot database ( https://www.uniprot.org/ , accessed on 9 November 2019).

Techniques: Sequencing

Journal: Pathogens

Article Title: Peptidases Are Potential Targets of Copper(II)-1,10-Phenanthroline-5,6-dione Complex, a Promising and Potent New Drug against Trichomonas vaginalis

doi: 10.3390/pathogens12050745

Figure Lengend Snippet: Docking pose with lower absolute energy (kcal·mol −1 ) and 3D interaction diagram of the compounds with the amino acids of TvMP50 and TvGP63 from Trichomonas vaginalis . ( A , E ) 1,10-phenanthroline, ( B , F ) phendione, ( C , G ) Ag-phendione, ( D , H ) Cu-phendione.

Article Snippet: The analysis was performed on a G2-XS Q-TOF Xevo mass spectrometer ® and the identification was based on the genome database of T. vaginalis ( T. vaginalis G3, G3; ATCC PRA-98, WGS project AAHC01000000) held on the ProteinLynx Global Server (PLGS) platform version 2.2.5 and Uniprot database ( https://www.uniprot.org/ , accessed on 9 November 2019).

Techniques:

Journal: Pathogens

Article Title: Peptidases Are Potential Targets of Copper(II)-1,10-Phenanthroline-5,6-dione Complex, a Promising and Potent New Drug against Trichomonas vaginalis

doi: 10.3390/pathogens12050745

Figure Lengend Snippet: ( A ) Docking pose with lower absolute energy (kcal·mol −1 ) and ( B ) 3D interaction diagram of the Cu-phendione with the amino acids of TvCP2 from Trichomonas vaginalis .

Article Snippet: The analysis was performed on a G2-XS Q-TOF Xevo mass spectrometer ® and the identification was based on the genome database of T. vaginalis ( T. vaginalis G3, G3; ATCC PRA-98, WGS project AAHC01000000) held on the ProteinLynx Global Server (PLGS) platform version 2.2.5 and Uniprot database ( https://www.uniprot.org/ , accessed on 9 November 2019).

Techniques:

Journal: Pathogens

Article Title: Peptidases Are Potential Targets of Copper(II)-1,10-Phenanthroline-5,6-dione Complex, a Promising and Potent New Drug against Trichomonas vaginalis

doi: 10.3390/pathogens12050745

Figure Lengend Snippet: Docking results predicted by AutoDock Vina: binding affinity, interaction type, amino acid residue, and geometric distance (Å) for TvMP50 of Trichomonas vaginalis with test compounds.

Article Snippet: The analysis was performed on a G2-XS Q-TOF Xevo mass spectrometer ® and the identification was based on the genome database of T. vaginalis ( T. vaginalis G3, G3; ATCC PRA-98, WGS project AAHC01000000) held on the ProteinLynx Global Server (PLGS) platform version 2.2.5 and Uniprot database ( https://www.uniprot.org/ , accessed on 9 November 2019).

Techniques: Binding Assay, Residue

Journal: Pathogens

Article Title: Peptidases Are Potential Targets of Copper(II)-1,10-Phenanthroline-5,6-dione Complex, a Promising and Potent New Drug against Trichomonas vaginalis

doi: 10.3390/pathogens12050745

Figure Lengend Snippet: Docking results predicted by AutoDock Vina: binding affinity, interaction type, amino acid residue, and geometric distance (Å) for TvGP63 of Trichomonas vaginalis with test compounds.

Article Snippet: The analysis was performed on a G2-XS Q-TOF Xevo mass spectrometer ® and the identification was based on the genome database of T. vaginalis ( T. vaginalis G3, G3; ATCC PRA-98, WGS project AAHC01000000) held on the ProteinLynx Global Server (PLGS) platform version 2.2.5 and Uniprot database ( https://www.uniprot.org/ , accessed on 9 November 2019).

Techniques: Binding Assay, Residue

Journal: Pathogens

Article Title: Peptidases Are Potential Targets of Copper(II)-1,10-Phenanthroline-5,6-dione Complex, a Promising and Potent New Drug against Trichomonas vaginalis

doi: 10.3390/pathogens12050745

Figure Lengend Snippet: Docking results predicted by AutoDock Vina: binding affinity, interaction type, amino acid residue, and geometric distance (Å) for TvCP2 of Trichomonas vaginalis with test compounds.

Article Snippet: The analysis was performed on a G2-XS Q-TOF Xevo mass spectrometer ® and the identification was based on the genome database of T. vaginalis ( T. vaginalis G3, G3; ATCC PRA-98, WGS project AAHC01000000) held on the ProteinLynx Global Server (PLGS) platform version 2.2.5 and Uniprot database ( https://www.uniprot.org/ , accessed on 9 November 2019).

Techniques: Binding Assay, Residue

Journal: Pathogens

Article Title: Peptidases Are Potential Targets of Copper(II)-1,10-Phenanthroline-5,6-dione Complex, a Promising and Potent New Drug against Trichomonas vaginalis

doi: 10.3390/pathogens12050745

Figure Lengend Snippet: Cu-phendione inhibits proteolytic activity in T. vaginalis . ( A ) Azocasein cleavage rate by parasites treated (12.5 μM) or not with Cu-phendione (CPh), pH 7.0, 37 °C for 90 min. ( B ) Cleavage of Phe-Arg substrate by parasite lysate at pH 5.0, 37 °C for 1 h. Results are expressed as arbitrary fluorescence units (AFU). ( C ) Cleavage of DNP-Pro-Leu-Gly-Met-Trp-Ser-Arg (MMP) substrate by parasite lysate at pH 9.0, 37 °C for 1 h. Results are expressed as arbitrary fluorescence units (AFU). CTL: Untreated control. FNT: 1,10-Phenanthroline. E-64: L-trans -epoxysuccinyl-L-leucylamido(4-guanidino) butane. (*) indicates statistically significant decrease (Student’s t- test; p < 0.05).

Article Snippet: The analysis was performed on a G2-XS Q-TOF Xevo mass spectrometer ® and the identification was based on the genome database of T. vaginalis ( T. vaginalis G3, G3; ATCC PRA-98, WGS project AAHC01000000) held on the ProteinLynx Global Server (PLGS) platform version 2.2.5 and Uniprot database ( https://www.uniprot.org/ , accessed on 9 November 2019).

Techniques: Activity Assay, Fluorescence, Control

Journal: Pathogens

Article Title: Peptidases Are Potential Targets of Copper(II)-1,10-Phenanthroline-5,6-dione Complex, a Promising and Potent New Drug against Trichomonas vaginalis

doi: 10.3390/pathogens12050745

Figure Lengend Snippet: Zymogram of the effect of Cu-phendione on T. vaginalis peptidases. Peptidase zymograms with gelatin after 24 h of incubation of ATCC 30236 isolate treated or not with Cu-phendione at MIC (12.5 μM). The region marked by the square was cut out of the zymogram for gel digestion. MW: Molecular weights in SDS-PAGE without gelatin.

Article Snippet: The analysis was performed on a G2-XS Q-TOF Xevo mass spectrometer ® and the identification was based on the genome database of T. vaginalis ( T. vaginalis G3, G3; ATCC PRA-98, WGS project AAHC01000000) held on the ProteinLynx Global Server (PLGS) platform version 2.2.5 and Uniprot database ( https://www.uniprot.org/ , accessed on 9 November 2019).

Techniques: Incubation, SDS Page

Journal: Pathogens

Article Title: Peptidases Are Potential Targets of Copper(II)-1,10-Phenanthroline-5,6-dione Complex, a Promising and Potent New Drug against Trichomonas vaginalis

doi: 10.3390/pathogens12050745

Figure Lengend Snippet: Peptidases from Trichomonas vaginalis found in the band demarcated in the zymogram ( Figure S1 ) and identified by mass spectrometry.

Article Snippet: The analysis was performed on a G2-XS Q-TOF Xevo mass spectrometer ® and the identification was based on the genome database of T. vaginalis ( T. vaginalis G3, G3; ATCC PRA-98, WGS project AAHC01000000) held on the ProteinLynx Global Server (PLGS) platform version 2.2.5 and Uniprot database ( https://www.uniprot.org/ , accessed on 9 November 2019).

Techniques: Mass Spectrometry, Molecular Weight, Ubiquitin Proteomics

Journal: Pathogens

Article Title: Peptidases Are Potential Targets of Copper(II)-1,10-Phenanthroline-5,6-dione Complex, a Promising and Potent New Drug against Trichomonas vaginalis

doi: 10.3390/pathogens12050745

Figure Lengend Snippet: Analysis of the mRNA expression of calpain, cathepsin L, cathepsin D, cathepsin B, Gp63, and MP50 relative to the reference gene ( DNATopII ) in T. vaginalis treated with Cu-phendione. Statistically significant differences: (*) p < 0.05; (**) p < 0.005; (****) p < 0.0001.

Article Snippet: The analysis was performed on a G2-XS Q-TOF Xevo mass spectrometer ® and the identification was based on the genome database of T. vaginalis ( T. vaginalis G3, G3; ATCC PRA-98, WGS project AAHC01000000) held on the ProteinLynx Global Server (PLGS) platform version 2.2.5 and Uniprot database ( https://www.uniprot.org/ , accessed on 9 November 2019).

Techniques: Expressing

Journal: Pathogens

Article Title: Peptidases Are Potential Targets of Copper(II)-1,10-Phenanthroline-5,6-dione Complex, a Promising and Potent New Drug against Trichomonas vaginalis

doi: 10.3390/pathogens12050745

Figure Lengend Snippet: LDH release by the HMVII and VERO cell lineages after contact with Trichomonas vaginalis a .

Article Snippet: The analysis was performed on a G2-XS Q-TOF Xevo mass spectrometer ® and the identification was based on the genome database of T. vaginalis ( T. vaginalis G3, G3; ATCC PRA-98, WGS project AAHC01000000) held on the ProteinLynx Global Server (PLGS) platform version 2.2.5 and Uniprot database ( https://www.uniprot.org/ , accessed on 9 November 2019).

Techniques: